Laboratory of  Human Molecular Genetics and Genomic Disorders
  The University of Western Ontario

This web interface provides a tool to predict the effects of sequence changes that alter mRNA splicing in human diseases. We designed the system to evaluate changes in splice site strength based on information theory-based models of donor and acceptor splice sites.

The resource has been updated and improved by its previous interation. It has been updated with the lastest genomic coordinates (HG19), the newest mRNA tables (UCSC), known SNPs (dbSNP135), and updated donor and acceptor weight matrices. In this iteration, we introduce a method to predict potential isoform structures and their relative abundance.

Watch a tutorial video describing how to use the web server and understand your results.

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December 18th, 2014 - Notice to ASSEDA Users: There will be a brief shutdown of the ASSEDA Server on Friday December 19th, at 11pm EST for system maintenance. This shutdown should take a maximum of 8 hours. We apologize for any inconvenience this might cause.

March 31, 2014 - Notice to ASSEDA Users: We have initiated a line of software products for prediction of functionally-significant, non-coding variants in complete genome or exome sequences. Comprehensive genome-scale analysis is now possible for mutations which completely or partially inactivate mRNA splice sites or activate cryptic splicing. The Shannon Human Splicing pipeline is now available on the web. It uses the same information theory-based binding site analysis available on this server, however genome-wide data can be examined. We have also developed, Veridical, which experimentally validates any in silico splicing mutation predictions, including but not limited to those generated by the Shannon Pipeline, by leveraging corresponding RNA-Seq data. The paper describing this software is published by F1000Research.



Peter Rogan, Ph.D.

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